We study the transient interactions that soluble proteins establish with lipid membranes. Our interest is to understand how the partial unfolding of the protein in the interface is related to binding and membrane translocation process. We study the conformational changes of proteins that bind to the lipid membrane and the changes in the thermal stability using UV absorption, fluorescence emission, FTIR, and circular dichroismand spectroscopy. We use Molecular Dynamics to simulate peptides and proteins in solution and bound to lipid membranes to study the forces involved in these process and the structures acquired by the polymer chain in the lipid membrane.